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size is signi cantly larger than the cell, negligible energy is transferred to the cell surface and the cell can be thought of as a freely rotating object in a relatively quiescent uid bounded by the dimensions of the eddy If the eddy size is on the same order as the cell, signi cant transfer of energy can take place, which can lead to damage Although estimates of the Kolmogorov microscale are useful in determining whether damage can occur, care should be taken in estimation of the stresses based on this theory First, the turbulence intensity in a bioreactor is not homogeneous, with almost 100 fold higher local energy dissipation rates found in the impeller region (Zhou and Kresta, 1996) The maximum time-averaged stresses occur in the trailing vortices behind the impeller blades.

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Many studies do not consider this heterogeneity and have used an average energy dissipation rate, which may not re ect the local peak values Second, Kolmogorov s theory does not shed light on the mechanisms of cell damage; it does not describe how the cell and a turbulent eddy may interact It is possible that the presence of cells may affect the uid ow eld, and estimates of velocity and pressure uctuations on the scale of the cell may not be accurate (Barresi, 1997), although at the typical 001 to 0001 volume fractions, this is unlikely to occur.

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Also, Reynolds stresses, which arise from a consideration of momentum transport in turbulence are commonly used to estimate stresses on cells (Prokop and Bajpai, 1992), do not have obvious meaning since they include eddy motions of all sizes, whereas only eddies of sizes comparable to the Kolmogorov microscale may be relevant for cell eddy interactions Another problem in the application of Kolmogorov s theory is that related to turbulence intermittency as discussed earlier, and further work is required to determine how intermittency affects cell uid interactions..

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